Welcome!
Promoting Resilience through Emerging Clinical and Evolving Determinants of Equity (PRECEDE) Biomarkers Laboratory
Dr. Gill’s Research Laboratory:
Blood based biomarkers to be analyzed will be undertaken by Dr. Gill who is a Professor at Johns Hopkins University. Her laboratory is approximately 1,800 square and is located in the School of Nursing. In her laboratory there are 3 full-time investigators, including a laboratory manager, as well as up to 4 fellows each year. Her lab has all the necessary equipment to function as a molecular biological laboratory, including centrifuges, a biological safety cabinet, balances, freezers and refrigerators. Freezers for sample storage are under freezer monitory systems and are fully functional and available for study samples.
Olink Technology:
Olink technology is a high-throughput proteomics platform that uses Proximity Extension Assay (PEA) technology to enable precise, multiplex protein biomarker analysis. By employing dual antibody binding and DNA-based detection, it offers high specificity and sensitivity, even in complex biological samples like plasma and serum. Olink’s applications span biomarker discovery, disease mechanism studies, and therapeutic development, with a focus on gaining insights into areas such as oncology, neurology, cardiovascular, and immunology. Its quantitative, reproducible data empowers researchers to identify and validate protein signatures across diverse disease states, advancing precision medicine and translational research.
Assays Available: Explore HT, Reveal, Target 48, Target 96, Flex, and Focus
Explore HT and Reveal assay kits utilize the NGS, while Target, Flex, and Focus use the Olink Signature Q100 for data generation. Equipment and Technology: PCR hood, F.A.S.T, Dragonfly, ProFlex PCR system, Verti96wThermocycler, Agilent Tapestation 4200, Olink Signature Q100
Chelsea Wagner
Lab Director
| Hours/Days Open | Hours/Days Staffed (if different) | Location |
| Monday - Friday 9am-4pm | Monday - Thursday 9am-3pm | 525 N. Wolfe St |
| Lab Room S005 | ||
| Baltimore, MD 21205 |
https://nursing.jhu.edu/faculty-research/research/centers/precede-biomarkers-laboratory/
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Chelsea Wagner |
Lab Director
|
chelseawagner@jhu.edu
|
525 N Wolfe St
|
|
| Lea Fortuno-Miranda |
Sr. Research Specialist
|
lfortun1@jh.edu
|
525 N Wolfe St
|
