iLab Organizer :: The Center for Proteomics Discovery

The Proteomics and Mass Spectrometry Facility has been renamed to the Center for Proteomics Discovery (CPD).

If you have any questions about the services provided at CPD, please try our CPD consultant AI at the link below. You can get most answers here. It also reads your input files (experimental plan, SDS-PAGE images, WB images, etc.) about your project.

Proteomics Center Consultant AI

If you need consultation, please contact "CPD@live.johnshopkins.edu".

Overview of Services

The Johns Hopkins University (JHU) School of Medicine (SOM) Center for Proteomics Discovery (CPD) assists investigators to identify and quantify proteins and their modifications that are differentially expressed in cells, tissues or body fluids, to track interactions with binding partners during changes in signal transduction, and to identify proteolytic cleavage sites and map post-translational modifications by providing the following proteomic services:

Consultation: Pre- and post-analysis.
Proteomics

Sample preparation: Buffer exchange; Column chromatography; Proteolytic digestion; Stable isotope labeling.
Protein Identification: Proteins in solution, in gel bands or spots, or in complex protein extracts.
Protein Modifications: A growing list of known and custom post translational modifications (PTMs) which include Acetylation, AMPylation, ADP ribosylation, citrullination, glycosylation, phosphorylation, proline hydroxylation, nitrosation, ubiquitination and novel cleavage sites, etc.
Protein Quantification: Relative quantification: Multi-dimensional protein identification technology (MuDPIT), isobaric mass tags (TMTs) or stable isotopic amino acids (SILAC); Targeted Quantification: Parallel (PRM) Reaction Monitoring, Internal Standard Triggered-Parallel Reaction Monitoring (IS-PRM), and Protein Concentration ("Absolute Quantification") using standard peptides (AQUA) or recombinant proteins (PSAQ).
High Resolution Mass Analysis: Accurate mass of intact proteins; Characterization protein modifications.
Single-cell proteome analysis: We can analyze single cells from cell culture or tissues.
Cell-type-specific proteome analysis: We can conduct cell-type-specific proteome analysis of tissues using antibody-mediated biotinylation (iCAB)
Spatial proteomics: We can conduct spatial analysis of tissue sections by combining laser capture microdissection and LC-MS/MS
Monoclonal antibody sequencing: We can conduct de novo sequencing of a monoclonal antibody.
Thermal Proteome Profiling for drug-binding protein identification: We can identify drug-binding proteins by measuring changes in protein stability when proteins bind a drug.
Single-molecule analysis using Quantum-Si: We have a walk-up instrument for protein sequencing, which can detect several tens of proteins from ~100 ng of protein sample.
Advanced Statistical/Bioinformatics Analysis: PCA analysis, Volcano plots, Ingenuity-based gene ontology pathway analysis; Bioinformatics consultation.
Machine Learning Analysis for biomarker discovery

Metabolomics/Lipidomics

Sample preparation: Preparation of metabolomics/lipidomics samples
Identification and quantification of metabolome/lipidome: Untargeted or targeted identification and quantification
Advanced Statistical/Bioinformatics Analysis
Machine Learning Analysis for biomarker discovery

Location

Director's Office: 709 Miller Research Building (MRB) & 512 Wood Basic Science Building (WBSB)
Associate Director's Office: 512 Wood Basic Science Building (WBSB)
Staff Offices: 509 and 510 Hunterian Building (HUNT)
Research Labs: 503 and 504 Wood Basic Science Building (WBSB)
MALDI Lab: 511 Hunterian (HUNT)
Phone Number: 410-614-6968

Hours

Operation: Monday through Friday, 9 am - 5 pm
Sample Drop off freezer at WBSB 503: available all week, all times

Sample Drop Off Protocol:
1. Label tubes with: Tube number, iLabs Service ID, Sample Name
2. Place samples in an empty numbered rack in the core’s -20C freezer located outside WBSB 503.
3. Take a picture of your samples in the freezer rack.
4. Upload the picture to your project in iLabs. No picture, can’t start project.

Shipping Address

Dr. Chan-Hyun Na
Center for Proteomics Discovery
Institute for Cell Engineering, HUNT 510
Johns Hopkins School of Medicine
725 N. Wolfe St.
Baltimore, MD 21205

Links and Resources

Contacts

Name	Role	Phone	Email	Location
Chan-Hyun Na, Ph.D.	Director	443-287-8631	chanhyun@jhmi.edu	MRB 709 & WBSB 512
Jodie Franklin, B.S.	Lab Manager	410-614-6968 ext 2	jfrankl1@jhmi.edu	Hunterian 510
Robert N. O'Meally, M.S.	Technical Director, Proteomics Specialist	410-614-6968 ext 2	romeall1@jhmi.edu	Hunterian 510
Joshua W. Smith, PhD	Research Associate	410-614-6968 ext 3	joshuasmith@jhu.edu	Hunterian 509
David Kirchner, M.S.	Proteomics Specialist	410-614-6968 ext 3	dkirchn3@jh.edu	Hunterian 509
Taekyung Ryu, Ph.D.	Associate Director	410-614-6968 ext 1	tryu3@jhmi.edu	WBSB 512

The Center for Proteomics Discovery