Welcome!
ALERT Important Information Regarding JHU iLab URL Change on 11/9/24
Please be aware that as of 8/3/2024 the URL for the Johns Hopkins University iLab website has changed to https://johnshopkins.ilab.agilent.com. The temporary URL re-direct will expire on 11/9/24. Therefore, it is important that you update your saved bookmarks to the new URL prior to this date. If you encounter any issues with logging into iLab with your JHED ID, please call the Johns Hopkins IT Help Desk at 410-516-HELP (410-516-4357) or submit a Johns Hopkins Help Desk self-service ticket online by accessing https://myit.jh.edu/apps/mfahelpticket.
The JHU EV & Nanoparticle Core Facility provides researchers with a full spectrum of services related to studies of extracellular vesicles (EVs) and other nanoparticles. EVs are increasingly studied not only in basic cell biology, but also as platforms for biomarkers and therapeutics. In the core, experimental design consultation will be available to ensure compliance with the international consensus standards in EV studies, the Minimal Information for Studies of EVs (MISEV), including in the area of sample collection and pre-processing. Several EV separation techniques are offered that can be done separately or in sequence: size exclusion chromatography (SEC) by Fast Protein Liquid Chromatography (FPLC) or gravity (Izon pre-packed columns with automated fraction collectors); tangential flow filtration (TFF); differential ultracentrifugation (dUC); density gradient ultracentrifugation (dgUC); and affinity separations.
The core offers a comprehensive range of EV characterization approaches using state-of-the-art technologies: resistive pulse sensing (RPS, Spectradyne), nanoparticle tracking analysis (NTA; NanoSight, ZetaView), nano-flow cytometry (NanoFCM), interferometric imaging plus immunophenotyping (SP-IRIS, ExoView/Leprechaun), and multiplexed ultrasensitive ELISA (Meso Scale Discoveries), and super-resolution microscopy (ONI). Importantly, several of these methods are suitable for cutting-edge single-particle phenotyping. More standard characterization methods such as Western blotting, ELISA, and protein/lipid/nucleic acid measurements will also be offered. Comprehensive molecular profiling (advice and/or services in combination with existing JH cores) will be available for proteins and RNA. Consultation services will also be available for how to investigate EVs after separation and characterization.
Kenneth Witwer, PhD, Director
Olesia Gololobova, PhD, MEng, Manager
| Hours | |||
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Monday - Friday 8:00 am-6:00 pm
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| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Olesia Gololobova |
For requests
|
ogololo1@jh.edu
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733 North Broadway Miller Research Building Baltimore, MD 21205
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