Description

The Macromolecular Biophysics Core provides researchers with access, training, and expert support for state-of-the-art instrumentation to characterize macromolecules, including Biacore SPR, mass photometry, microscale thermophoresis (MST), size-exclusion chromatography with multi-angle light scattering (SEC-MALS), dynamic light scattering (DLS), isothermal titration calorimetry (ITC), differential scanning calorimetry (DSC), and robotics for macromolecular crystallization.

Overview of Services

The Macromolecular Biophysics (MacBio) Core offers access and training to a range of instruments that can be used to biophysically characterize biological macromolecules. These resources were previously available only to a few labs at the SOM, but the core now centralizes them and provides training to all JHU researchers. Some of the available instruments include Biacore 8K (SPR), Refeyn Two-MP Mass Photometer (MP), NanoTemper NT-11.5 for MST, Agilent HPLC coupled to Wyat's Heleos II and T-REX detectors for SEC-MALS, Titan for DLS , Malvern's  ITC200 for ITC,  nanoDSC  for Differential Scanning Calorimetry. The MacBio Core also offers facilities and instruments for macromolecular crystallization, such as a Mosquito-LCP crystallization robot and a RockImager automatic imaging and constant temperature room. These resources are overseen to ensure that inexperienced users receive proper training and guidance.

Leadership

Cynthia Wolberger

Location and hours of operation

Hours	Location
10:00 AM - 4 PM Monday to Friday	725 N wolfe st  Wood Basic Science Building 623      Baltimore, MD,21205

Links and Resources

1. https://biophysics.med.jhmi.edu/macbio/

Contacts

Name	Role	Phone	Email	Location
Mario A Bianchet, PhD	Director	443 5917583	mbianch1@jh.edu	WBSB608B

Available Equipment and Resources

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