Integrated Imaging Center

Overview of Services

The Integrated Imaging Center (IIC) is a large bio-imaging facility based at theJohns Hopkins Homewood Campus. We encompass light an electron microscopy as well as flow cytometry, gel imagers and bioprinting. The IIC currently serves more than 80 laboratories and >250 users in the greater Baltimore area. The Center is based in both the Krieger School of Arts & Sciences and the Whiting School of Engineering, with more than 30 instruments in 4 buildings.

The center is Directed by Ian Dobbie, a Research Professor in Biology, who has 20+ years of experience in optical microscopy, specializing in building super resolution imaging systems to answer diverse biomedical research questions. The IIC is managed by Erin Pryce. A graduate of Ithaca College, Erin is an expert in all aspects of fluorescence LM (including confocal, deconvolution, FRET/FRAP/FCS) and transmission electron microscopy. Hanhvy Bui is the manager of our Homewood Flow Cytometry Resource, a core facility offering flow cytometry training and cell sorting services. Yuan Cai is a Research Specialist 2 who oversees the microscopy suite in Dunning Hall. She is also our Lightsheet specialist and an expert in Imaris a leading visualization package.

The center was originally established as the Advanced Microscopy Facility (AMF) and was devoted exclusively to LM techniques; it was initially directed by Dr. William Busa and later by Dr. Michael Edidin. In 1998, the AMF was incorporated into a newly created EM Facility under the direction of J. Michael Macaffery to form the present IIC.

Leadership

Director:  Ian Dobbie, Ph.D.

Location and hours of operation

24/7 access for Trained Users 

The Integrated Imaging Center (IIC) is a Homewood campus core facility located in Croft, Dunning, Levi, Mudd and Macaulay  (see numbers 27, 18, 11 and 12 circled in yellow on map), and serves the diverse academic and research interests of the students and faculty in the Whiting School of Engineering and the Krieger School of Arts and Sciences, as well as the Hopkins-wide community. Our Center comprises flow cytometry and cell sorting, fluorescence/light microscopy, and electron microscopy, including conventional, analytical, and cryogenic TEM. The IIC emphasizes the complementary use of these tools for the purpose of investigating cellular/subcellular structure and function, nanobiotechnology, and materials and engineering. The Center is housed in ~5000 sq.ft. suite of rooms, and is readily available to all interested University and non-University users. Additionally, our substantial computer resources are easily accessed by authorized users (campus wide and elsewhere) via the Internet.

Directions: From I-83 North or South take the W 28th Street exit and go east; proceed to Remington Ave. and turn left. Go north to the second stoplight and turn right onto Wyman Park Dr; at the stop sign, turn left onto San Martin Dr. Olin Hall will be ahead on your left; after a couple of lazy 'S' turns, pass under the foot bridge that crosses San Martin drive and turn right into the West Gate entrance to Hopkins. Proceed to and through the next stop sign; drive directly towards the building facing you (Levi Building). Veer slightly left and park in any one of three spaces labeled 'IIC Visitors.' Place parking pass on your driver's side dashboard; walk southwest and up the steep staircase next to the New Chemistry Building (#10) and at the top veer southeast towards Dunning Hall (#18). Enter Dunning, turn right, and ring the door bell on the east wall next to the IIC entrance. If no one appears to let you in within a couple of minutes, please call 410-516-8042 or 410-516-3321.

Links and Resources

• Integrated Imaging Center
• Instrumentation
• Techniques and Methodologies
• Protocols
• General fluorophore filter/spectra information
• System Fluorophore Information
• Literature, Guides, & Tutorials

Contacts