Welcome!
ALERT Please be aware that as of 8/3/2024 the URL for the Johns Hopkins University iLab website will be changing to https://johnshopkins.ilab.agilent.com. Any previously saved bookmarks for iLab should be updated accordingly. If you encounter any issues with logging into iLab with your JHED ID, please call the Johns Hopkins IT Help Desk at 410-516-HELP (410-516-4357) or submit a Johns Hopkins Help Desk self-service ticket online by accessing https://myit.jh.edu/apps/mfahelpticket.
(hide this warning on this page)
Our aim is to provide cost-effective and time-efficient access to cutting-edge genomic technologies and expert assistance with experimental design and data analysis. The Core has been in continuous operation since 2002 and has supported research projects for more than 467 principal investigators, 1097 individual researchers, and processed more than 36,000 samples (as tallied in April 2016). We offer sample preparation and analysis using Next Generation Sequencing technologies, and qPCR. We also offer high throughput DNA, Chromatin, and RNA shearing using the Covaris E220 system. Our lab is equipped with the Illumina NextSeq 500, MiSeq, and iSeq next-generation sequencer for short reads. We work closely with the ECGC and GRCF to enable our clients access to the NovaSeq for deeper sequencing requirements. We ensure a full-service experience for our clients, starting with project design, all the way through publication. We encourage our clients to meet with us before beginning any projects to ensure the most accurate and efficient results. From nucleic acid extraction through analysis, we are your one-stop-shop.
Seth Blackshaw, Ph.D., Faculty Director
MRB Room 339
Email: sethblackshaw@gmail.com
Emanuele Palescandolo, Ph.D., Director
MRB Room 359
Email: epalesc1@jh.edu
BJ Savoy, MBA, CPA, Finance Director
410.502.3071
Email: bjsavoy@jhu.edu
| Hours | Location |
|
Hours Open: Monday - Friday 8:30am-5:00pm Hours Fully Staffed: Monday - Friday 8:30am-5:00pm |
733 N Broadway |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Seth Blackshaw, PhD |
Scientific Director
|
sethblackshaw@gmail.com
|
MRB Room 335
|
|
| Jasmeet Sethi, PhD |
Bulk Sequencing specialist
|
443-287-8852
|
jsethi1@jhmi.edu
|
MRB Room 355
|
| Conover Talbot Jr., BS |
Bioinformatician
|
410-614-0443
|
cct@jhmi.edu
|
MRB Room 353
|
| Jongseok Lee, PhD |
Single Cell Sequencing Specialist (DNA)
|
jlee243@jhmi.edu
|
MRB Room 360
|
|
| BJ Savoy, MBA, CPA |
Finance Director
|
410-502-3071
|
bjsavoy@jhu.edu
|
|
| Emanuele Palescandolo |
Director
|
410-502-6658
|
epalesc1@jh.edu
|
MRB Room 359
|
| Russ Hughes |
Spatial Txomics Specialist
|
410-614-0443
|
russ@jhu.edu
|
MRB Room 353
|
| Lauren Yum |
Single Cell Specialist
|
lyum1@jh.edu
|
MRB Room 353
|
| Price list |
| ► Data Analysis (2) | |||
| Name | Description | Price | |
|---|---|---|---|
| Data Analysis | Inquire | ||
| Ingenuity |
Usage of Ingenuity Pathway Analysis. This service requires user to register an account with Ingenuity. |
Inquire | |
| ► Library Prep (25) | |||
| Name | Description | Price | |
| 16S rRNA Library prep (1-16 samples) |
16S library prep, V3/V4, 1-16 samples |
Inquire | |
| 16S rRNA Library prep (17-48 samples) |
Metagenomic sequencing targeting 16s rRNA variable region 3 and 4. 17-48 samples. |
Inquire | |
| 16s rRNA Library prep (49+ samples) |
Metagenomic sequencing targeting 16s rRNA variable region 3 and 4. |
Inquire | |
| ATAC-seq library prep | Inquire | ||
| DNA/ChIP seq library preparation (96+) | Inquire | ||
| DNA/ChIPseq library preparation | Inquire | ||
| Nextera | Inquire | ||
| Nextera (48+) | Inquire | ||
| Nextera (8+) | Inquire | ||
| small RNAseq library prep | Inquire | ||
| SMART-seq mRNA LP library prep (non-stranded) (8+) | Inquire | ||
| SMART-seq mRNA v4 library prep (non-stranded) | Inquire | ||
| SMART-seq mRNA v4 library prep (non-stranded) (24+) | Inquire | ||
| SMART-seq mRNA v4 library prep (non-stranded) (96+) | Inquire | ||
| SMART-seq Total RNA v3 library prep | Inquire | ||
| SMART-seq Total RNA v3 library prep (24+) | Inquire | ||
| SMART-seq Total RNA v3 library prep (8+) | Inquire | ||
| SMART-seq Total RNA v3 library prep (96+) | Inquire | ||
| Stranded mRNA Seq library prep with UDI | Inquire | ||
| Stranded mRNA Seq library prep with UDI (8+) | Inquire | ||
| Stranded mRNA Seq library prep with UDI (96+) | Inquire | ||
| Stranded Total RNA Seq library prep with UDI | Inquire | ||
| Stranded Total RNA Seq library prep with UDI (24+) | Inquire | ||
| Stranded Total RNA Seq library prep with UDI (8+) | Inquire | ||
| Stranded Total RNA Seq library prep with UDI (96+) | Inquire | ||
| ► Nucleic Acid Extraction (6) | |||
| Name | Description | Price | |
| Blue Pippin Size selection |
QC charges separate |
Inquire | |
| DNA extraction (12+) | Inquire | ||
| Nucleic Acid cleanup | Inquire | ||
| Nucleic Acid cleanup RUSH | Inquire | ||
| RNA extraction | Inquire | ||
| RNA extraction (12+) | Inquire | ||
| ► Parse (2) | |||
| Name | Description | Price | |
| Parse WT |
Parse single cell RNA combinatorial barcoding, up to 100K cells, up to 48 samples |
Inquire | |
| Parse WT library prep only | Inquire | ||
| ► Quality Control (9) | |||
| Name | Description | Price | |
| QC only |
Do NOT complete for single cell sample or library QC. Please complete Single Cell sequencing request for all single cell library QC and/or sequencing. |
Inquire | |
| QC only (12+) | Inquire | ||
| QC with qPCR |
$22 sample |
Inquire | |
| QC with qPCR (12+) |
Cost per sample |
Inquire | |
| QC with qPCR (RUSH) |
Cost per sample |
Inquire | |
| QC with qPCR (RUSH) (12+) | Inquire | ||
| qPCR only | Inquire | ||
| RUSH QC only | Inquire | ||
| RUSH QC only (12+) | Inquire | ||
| ► Sequencing (22) | |||
| Name | Description | Price | |
| iSeq 300 | Inquire | ||
| MiSeq 150 | Inquire | ||
| MiSeq 300 | Inquire | ||
| MiSeq 50 | Inquire | ||
| MiSeq 500 | Inquire | ||
| MiSeq 600 | Inquire | ||
| MiSeq Micro 300 | Inquire | ||
| MiSeq Nano 300 | Inquire | ||
| MiSeq Nano 500 | Inquire | ||
| NextSeq 500 High 150 |
NextSeq 500 sequencing using High Output 150 cycle reagents for single 150bp reads, or paired 75bp reads. Yield up to 400 million single/paired reads. |
JHU Internal
$4,100.00
each
|
|
| NextSeq 500 High 300 |
NextSeq 500 sequencing using High Output 300 cycle reagent kit for paired 150bp reads. Yield up to 400 million paired reads. |
JHU Internal
$5,800.00
each
|
|
| NextSeq 500 High 75 |
NextSeq 500 sequencing using High Output 75 cycle reagent kit for single 75bp reads. Yield up to 400 million reads. |
JHU Internal
$2,700.00
each
|
|
| NextSeq 500 Mid 300 (151-300bp) |
NextSeq 500 sequencing using Mid Output 300 cycle reagent kit for 300bp reads (any configuration). Yield upto 130 Million paired reads. |
Inquire | |
| NovaSeq S2 200 cycle (4.1B reads) | Inquire | ||
| NovaSeq S2 300 cycle (4.1B reads) | Inquire | ||
| NovaSeq S4 10X - 8 lanes - (1.25 B reads/lane) |
External - Academic
$9,600.00
each
External - Fast Forward/Startup $9,696.00 each |
||
| NovaSeq S4 200 cycle (10B reads) | Inquire | ||
| NovaSeq S4 200 XP | Inquire | ||
| NovaSeq S4 300 cycle (10B reads) | Inquire | ||
| NovaSeq SP 100 cycles (FC) | Inquire | ||
| NovaSeq SP 200 cycle (800M reads) | Inquire | ||
| NovaSeq X - 300 cycles - 1 lane - 10B FC (1,25 B reads/lane) | Inquire | ||
| ► Shearing (4) | |||
| Name | Description | Price | |
| Covaris shearing | Inquire | ||
| Covaris shearing (10+) | Inquire | ||
| gTube Shearing | Inquire | ||
| gTube Shearing (10+) | Inquire | ||
| ► Single Cell ATAC-seq (4) | |||
| Name | Description | Price | |
| 10X ATAC (2-7samples) | Inquire | ||
| 10X ATAC-seq | Inquire | ||
| 10X ATAC-seq (8+ samples) | Inquire | ||
| 10X ATAC-seq with Nuclei Isolation | Inquire | ||
| ► Single Cell DNA - MissionBio (2) | |||
| Name | Description | Price | |
| MissionBio DNA+Protein library prep | Inquire | ||
| MissionBio sample prep |
MissionBio DNA library prep. Reagents provided by MissionBio. |
Inquire | |
| ► Single Cell Multiome (10X ATAC-GEX) (3) | |||
| Name | Description | Price | |
| 10X multiome library prep (1st sample) | Inquire | ||
| 10X multiome library prep (samples 2-7) | Inquire | ||
| 10X multiome library prep (8+) | Inquire | ||
| ► Single Cell Sequencing (14) | |||
| Name | Description | Price | |
| 10X 3' HT | Inquire | ||
| 10X 5' HT | Inquire | ||
| 10X 5' library prep-1st sample | Inquire | ||
| 10X 5' library prep-samples 2-7 | Inquire | ||
| 10X 5' VDJ enrichment |
T cell or B cell enrichment and library prep performed alongside 5' gene expression library. |
Inquire | |
| 10X ADT library (CITE-seq) | Inquire | ||
| 10X Genomics Single Cell mRNA Library prep (8+) | Inquire | ||
| 10X Genomics Single Cell mRNA Library prep-1st sample |
10x Genomics Single cell mRNA sequencing to analyze 500-10,000 cells |
Inquire | |
| 10X Genomics Single Cell mRNA Library prep-samples 2-7 | Inquire | ||
| 10X HT chip recovery cost | Inquire | ||
| 10X Single cell (labor only) | Inquire | ||
| CellRanger | Inquire | ||
| Custom reference genome creation | Inquire | ||
| Hashtag/MULTI-seq library prep | Inquire | ||
| ► Spatial Transcriptomics (4) | |||
| Name | Description | Price | |
| Visium HD |
Price per run (2 slides) |
Inquire | |
| Visium FFPE Package |
Includes reagents, library pre, sequencing & SpaceRanger Sectioning, staining and imaging NOT included |
Inquire | |
| Visium spatial fresh/frozen assay |
Library prep only Does not include cDNA amplification or QC |
Inquire | |
| Visium spatial fresh/frozen with cDNA amp |
Includes cDNA cycle number determination, amplification, QC and library prep |
Inquire | |
