Welcome!
ALERT Important Information Regarding JHU iLab URL Change on 11/9/24
Please be aware that as of 8/3/2024 the URL for the Johns Hopkins University iLab website has changed to https://johnshopkins.ilab.agilent.com. The temporary URL re-direct will expire on 11/9/24. Therefore, it is important that you update your saved bookmarks to the new URL prior to this date. If you encounter any issues with logging into iLab with your JHED ID, please call the Johns Hopkins IT Help Desk at 410-516-HELP (410-516-4357) or submit a Johns Hopkins Help Desk self-service ticket online by accessing https://myit.jh.edu/apps/mfahelpticket.
GRCF Upcoming Events
The Genetic Resources Core Facility (GRCF) is a Johns Hopkins service center providing research expertise, products, and services for the study of the human genome. We strive to streamline services such as sample collection, cell line establishment, cell line authentication, cryogenic storage, DNA isolation, oligo and gene synthesis, methylation testing, both Sanger and next-gen sequencing as well as genotyping from handfuls to millions of variants. Bioinformatics support for sequencing projects is also available. The GRCF can help with project design and custom assay development for DNA and RNA based studies.
We are a part of Johns Hopkins Genomics and function as three groups: the Core Store, the Cell Center & BioBank and DNA Services. The DNA Services group encompasses the DNA Analysis Facility, the Nucleic Acid Technogies group, the High Throughput Sequencing group and the Bioinformatics group. This site is for ordering most of the services offered by the DNA Services unit. Sanger Seqeuncing and next-gen sequencing, may be ordered on our Genesifter server. The Cell Center & Biorepository and the Core Store maintain separate ordering sites.
Roxann Ashworth, MHS (DNA Analysis Facility)
Email: rashwor2@jhmi.edu
Phone: 410-614-0702
Location: Blalock 1004
Liliana Florea, PhD (Bioinformatics)
Email: florea@jhu.edu
Location: MRB 453 (Primarily works remotely)
Alejandra Gutierrez, PhD (Nucleic Acid Technologies)
Email: mgutie25@jhmi.edu
Phone: 410-614-3830
Location: 725 N. Wolfe Street, PCTB G20, Baltimore, MD 21205
David Mohr (High Throughput Sequencing)
Email: dwmohr@jhmi.edu
Phone: 667-208-7274
Location: 1812 Ashland, 2nd floor (Primarily works remotely)
General questions should be addressed to the DNA services contact point: customorders@jhmi.edu; 410-955-2836.
General Lab contact - Blalock Location
Email: customorders@jhmi.edu
Phone: 410-955-2836
Oligo order questions - Blalock Location
Email: customorders@jhmi.edu
Phone: 410-955-2836
General Lab contact - PCTB location
Email: faf@jhmi.edu
Phone: 443-287-7948
Location: 600 N. Wolfe St. Blalock 1004. Baltimore, MD 21287
Hours: Monday - Friday : 7am - 4:30pm
Location: 725 N. Wolfe Street, PCTB G20, Baltimore, MD 21205
Hours: Monday - Friday: 8:30am - 4:30pm
Location: 1812 Ashland St., Baltimore, MD 21205
Hours: Monday - Friday: 8 am - 4pm
Samples should be dropped off at the appropriate location or in one of our drop boxes. High Throughput Sequencing sample drop off must be arranged prior to drop-off. Please contact David Mohr.
Remote drop off locations:
“……….[insert the name of the service provided here]…………. was conducted at the Genetic Resources Core Facility, RRID:SCR_018669, Johns Hopkins University Department of Genetic Medicine, Baltimore, MD.”
If your journal is asking for RRIDs for materials and instrumentation used in your experiments, please reach out to us.
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| General Lab contact |
Blalock location
|
410-955-2836
|
customorders@jhmi.edu
|
Blalock 1004
|
| General Lab contact |
PCTB location
|
443-287-7948
|
faf@jhmi.edu
|
PCTB G20
|
| Oligo order questions |
Blalock location
|
410-955-2836
|
customorders@jhmi.edu
|
Blalock 1004
|
| Roxann Ashworth |
Co-Director
|
410-614-0702
|
rashwor2@jhmi.edu
|
Blalock 1004
|
| Alejandra Gutierrez |
Co-Director
|
410-614-3830
|
mgutie25@jhmi.edu
|
PCTB G20
|
| Breon Beech |
Lab Manager, Blalock
|
410-955-2836
|
bbeech@jhmi.edu
|
Blalock 1004
|
| David Mohr |
Co-Director
|
667-208-7274
|
dwmohr@jhmi.edu
|
Remote/1812 Ashland
|
| Liliana Florea |
Co-Director
|
florea@jhu.edu
|
Remote/MRB 453
|
| Service list |
| ► Bisulfite Conversion (1) | |||
| Name | Description | Price | |
|---|---|---|---|
| Bisulfite Conversion. Zymo column | Inquire | ||
| ► Digital PCR (1) | |||
| Name | Description | Price | |
| Digital PCR - 26k partitions | Inquire | ||
| ► Genotyping - Illumina - GWAS (9) | |||
| Name | Description | Price | |
| CytoSNP-850K - No Pretesting (93 or fewer Samples) |
Full service includes:
|
Inquire | |
| CytoSNP-850K - No pretesting (94-2,000 Samples) |
This service includes:
|
Inquire | |
| Global Diversity Array - Full Service (5000+ samples) |
Full service includes:
|
Inquire | |
| Global Diversity Array - Full Service (94-5000 samples) |
Full service includes:
|
Inquire | |
| Global Diversity Array - No Pretesting (93 samples) |
Full service without Pretesting includes:
|
Inquire | |
| Global Diversity Array - No Pretesting (94-5000) |
Full service without Pretesting includes:
|
Inquire | |
| Global Screening Array - Full service (94-2000 samples) |
Full service includes:
|
Inquire | |
| Global Screening Array - No pretesting (93 Samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
| Global Screening Array - No Pretesting (94-2000) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
| ► Genotyping: Non-Human (1) | |||
| Name | Description | Price | |
| Mouse GIGAmuga - Full Service (84-2000 samples) | Inquire | ||
| ► Genotyping: Specialized Content Options (1) | |||
| Name | Description | Price | |
| Human QC Array - no genotypes returned |
This service will test your samples to see if they are suitable for running on an Illumina array. |
Inquire | |
| ► Methylation Array (1) | |||
| Name | Description | Price | |
| FFPE restore |
This special process repairs highly fragmented DNA (such as from FFPE or CF DNA) so that it is usuable on any Illumina array. |
Inquire | |
| ► Pipette Services (3) | |||
| Name | Description | Price | |
| 12 Channel Multi-Channel Calibration |
For pipettes with 12 channels. |
Inquire | |
| 8 or fewer Channel Multi-channel Pipette Calibration |
For 8 channel or less pipettes. |
Inquire | |
| Single Channel Pipette Calibration | Inquire | ||
| ► Sequencing (2) | |||
| Name | Description | Price | |
| Oxford Nanopore Library prep |
Service for ONT library prep |
Inquire | |
| Oxford Nanopore Sequencing |
Service for ONT sequencing. |
Inquire | |
| ► Taqman Open Array Gene Expression chips (8) | |||
| Name | Description | Price | |
| Human Barcode | Inquire | ||
| Taqman OpenArray Custom Chip processing Costs - 16 assays |
The processing costs and chip costs are billed separately. This charge is for the processing costs, which vary depending on the type of chip and number of chips processed. Price shown is for 10 chips, price will be adjusted based on actual number of chips run. |
Inquire | |
| Taqman OpenArray Custom Chip processing Costs - 56 or more assays |
The processing costs and chip costs are billed separately. This charge is for the processing costs, which vary depending on the type of chip and number of chips processed. Price shown is for 20 chips, price will be adjusted based on actual number of chips run. |
Inquire | |
| Taqman OpenArray Custom Genotyping Chips - 12 SNPs |
Minimum order 10 chips, 144 samples per chip |
Inquire | |
| Taqman OpenArray Custom Genotyping Chips - 180 SNPs |
Minimum order 60 chips, 16 samples/chip |
Inquire | |
| Taqman OpenArray Genotyping Chips - 26 SNPs |
Minimum order 10 chips, 96 samples/chip. |
Inquire | |
| Human MicroRNA chip | Inquire | ||
| Mouse Inflammation Chip (1 Chip) | Inquire | ||
