Purchase of a 9 x 9 Cryo Storage Box, suitable for long term storage.

Lymphocyte isolation and cryopreservation. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells.Database entry for all aliquots.

Yearly Storage per vial

July 1- June 30 coverage 

Total charge initiated in July for investigators with less than 500 samples.

FAIL Attempt for expansion after attempts at service request completion are made 

Fresh WB Transformation and T25 flask. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells. Transformation of fresh lymphocytes with Epstein-Barr virus.Cryopreservation of 2 vial of (transformed) lymphoblasts @5x10E6cells. Database entry for all aliquotes. Allow 4 weeks for completion.

Cost per card

Fresh WB Transformation and hold. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6 cells. Transformation of fresh lymphocytes with Epstein-Barr virus. Cryopreservation of 4 vials (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow4-5 weeks for completion.

PicoGreen as assessed in FAF core.

Set up cost for Cell Line Distribution when 5-9 distribution requests per month are expected

Set up cost for Cell Line Distribution when fewer than 5 distribution requests per month are expected

Set up cost for Cell Line Distribution when 10-19 distribution requests per month are expected

Growth of culture for 14 days with visual inspection of contamination

User and PI must sign CryoCart/Cryogenic Liquids User Responsibility Agreement prior to use of CryoCart.

Fresh WB Transformation with bulk growth. Lymphocyte separation. Cell count.Viability determination. Cryopreservation of whole blood (1ml). Cryopresarvation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells.Transformation of fresh lymphocytes with Epstein-Barr virus. Bulk growth (1x10E8) of transformed cells. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 6-8 weeks for completion.

DNA Isolation by FAF

Customers: Use Dry Ice Request Form at top of page for purchasing

Dry Ice, price per 1 pound

Isolation and cryopreservation of plasma. Plasma is separated by centrifugation, recovered in a sterile manner. Cryopreservation of vials (0.5 -1 ml/vial), first 1-5 aliquots included in price (additional vials $1.00/vial), and banked at -80C (additional storage charges apply). Database entry for all aliquots.

Purchase of Envelope + Dry Ice for Customer Biospecimen Transport

Isolation and cryopreservation of sera. Sera is separated by centrifugation, recovered in a sterile manner.  Cryopreservation vial(s) (0.5 -1 ml/vial) vials, first 1-5 aliqots included in price (additional vials $1.00/vial), and banked at -80C (additional storage charges apply). Database entry for all aliquots.

FedEx Charges

Establish primary cells from skin biopsy.  Prepare frozen stocks 4 vials @1x10E6 up to 3x10E6 cells each of established cell lines.  Includes cell count and viability and Mycoplasma test of initial media containing the biopsy.

FAIL Attempt for Transformation after three attempts are made (or less if requested by researcher) to complete transformation. 

Cell culture expansion of established cell line, cryopreservation of replacement vial @5e6 cells/ml and Cell Pellet Preparation. Culture to 10e8 cells suitable for DNA analysis.

Growth of an established adherent cell line in a T175 flask

Growth of an established adherent cell line in a T25 flask

Growth of an established adherent cell line in a T75 flask

Growth of established cell lines in tissue culture flasks. Price per vial 

Growth of established suspension cell lines in T25 flask

Growth of established suspension cell lines in a T75 flask

Preparation of cell pellet (100,000,000 cells)

Subsequent bulk growth. Bulk growth (1x10E8 cells) of a transformed established cell line. Cryopreservation of cell pellet or 20 vials of transformed lymphoblasts. Allow approximately 2-3 weeks for completion.

Subsequent growth. T25 flask (5x10E6 cells) of a transformed cell line. Allow approximately 3-5 days for completion. (Replacement of initial vial)

Subsequent transformation and T25 flask. Transformation of frozen lymphocytes with Epstein-Barr virus. Cryopreservation of 2 vial of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 4 weeks for completion.

Subsequent transformation with bulk growth. Transformation of cryopreserved lymphocytes with Epstein-Barr virus.Bulk growth (1x10E8) of transformed. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquotes. Allow approximately 6-8 weeks for completion.

Subsequent transformation and hold. Transformation of frozen lymphocyteswith Epstein-Barr virus. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 4-5weeks for completion.

Prepare frozen stocks @ 5x10E6 cells each of established cell lines.

Thaw and transformation of lymphocytes w/EBV. Culture to 5x106 cells/ml, suitable for cryopreservation of 2 vial transformed lymphocytes. Allow 4 weeks for completion.

All services listed in (G), plus 2 additional cryopreserved vials of transformed lymphocytes (at 5x106 cells/ml) (Total 4).  Allow 4-5 weeks for completion.

All service in (H), plus culture to 1x108 cells/ml suitable for DNA analysis (cell pellet).  Allow 6-8 weeks for completion.

Specimen contaminated upon receipt

Culture suitable for T25 pick up and cryopreservation of 1 replacement vial (@ 5x10^6 cells/ml).  Allow 3-5 for completion.

Culture to 1x108 cells/ml suitable for DNA analysis (cell pellet) and cryopreservation of 1 replacement vial (@5x10^6 cells/ml).  Allow 2-3 weeks for completion.

Generation of labels based on File

Package UN3373 Large 60lb Box 24"x16"x16"

Please email biorepository@jhmi.edu to inquire about purchasing

Package UN3373 Medium 20lb Box 14"x14"14"

Please email biorepository@jhmi.edu to inquire about purchasing

Cell line deposit: 5 lines, no distribution

Monocyte isolation and cryopreservation. Lymphocyte & Monocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed monocytes @5x10E6cells. Database entry for all aliquots

Mycoplasm Test after completion

Quarterly storage per vial

For Investigators with >500 samples

Isolation of RNA from cultured cells by FAF

Package UN3373 Small 6lb Box 11"x9"x7"

Please email biorepository@jhmi.edu to inquire about purchasing

Defined dates and times for patient sample processing: including technician monitoring of critical processing time points, refrigerated centrifuge, and aliquoting on ice with snap freezing.

Disposal with Certificate of Discard

Administrative form fee for transfer of sample aliquots from Originating Principal Investigator to a recipient PI (JHMI or outside).

Supplies needed for modified protocol

Retrieval of specimens under vial rate.

WorldCourier Charges

Administrative form fee for transfer of sample aliquots from Originating Principal Investigator to a recipient PI (JHMI or outside).

Generation of labels based on File

Lymphocyte isolation and cryopreservation. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells.Database entry for all aliquots.

Fresh WB Transformation and T25 flask. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells. Transformation of fresh lymphocytes with Epstein-Barr virus.Cryopreservation of 2 vial of (transformed) lymphoblasts @5x10E6cells. Database entry for all aliquotes. Allow 4 weeks for completion.

Fresh WB Transformation and hold. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6 cells. Transformation of fresh lymphocytes with Epstein-Barr virus. Cryopreservation of 4 vials (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow4-5 weeks for completion.

Fresh WB Transformation with bulk growth. Lymphocyte separation. Cell count.Viability determination. Cryopreservation of whole blood (1ml). Cryopresarvation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells.Transformation of fresh lymphocytes with Epstein-Barr virus. Bulk growth (1x10E8) of transformed cells. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 6-8 weeks for completion.

Isolation and cryopreservation of plasma. Plasma is separated by centrifugation, recovered in a sterile manner. Cryopreservation of vials (0.5 -1 ml/vial), first 1-5 aliquots included in price (additional vials $1.00/vial), and banked at -80C (additional storage charges apply). Database entry for all aliquots.

Isolation and cryopreservation of sera. Sera is separated by centrifugation, recovered in a sterile manner.  Cryopreservation vial(s) (0.5 -1 ml/vial) vials, first 1-5 aliqots included in price (additional vials $1.00/vial), and banked at -80C (additional storage charges apply). Database entry for all aliquots.

Thaw and transformation of lymphocytes w/EBV. Culture to 5x106 cells/ml, suitable for cryopreservation of 2 vial transformed lymphocytes. Allow 4 weeks for completion.

All services listed in (G), plus 2 additional cryopreserved vials of transformed lymphocytes (at 5x106 cells/ml) (Total 4).  Allow 4-5 weeks for completion.

All service in (H), plus culture to 1x108 cells/ml suitable for DNA analysis (cell pellet).  Allow 6-8 weeks for completion.

Culture suitable for T25 pick up and cryopreservation of 1 replacement vial (@ 5x10^6 cells/ml).  Allow 3-5 for completion.

Culture to 1x108 cells/ml suitable for DNA analysis (cell pellet) and cryopreservation of 1 replacement vial (@5x10^6 cells/ml).  Allow 2-3 weeks for completion.

Monocyte isolation and cryopreservation. Lymphocyte & Monocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed monocytes @5x10E6cells. Database entry for all aliquots

Growth of culture for 14 days with visual inspection of contamination

Establish primary cells from skin biopsy.  Prepare frozen stocks 4 vials @1x10E6 up to 3x10E6 cells each of established cell lines.  Includes cell count and viability and Mycoplasma test of initial media containing the biopsy.

Cell culture expansion of established cell line, cryopreservation of replacement vial @5e6 cells/ml and Cell Pellet Preparation. Culture to 10e8 cells suitable for DNA analysis.

Growth of an established adherent cell line in a T175 flask

Growth of an established adherent cell line in a T25 flask

Growth of an established adherent cell line in a T75 flask

Growth of established cell lines in tissue culture flasks. Price per vial 

Growth of established suspension cell lines in T25 flask

Growth of established suspension cell lines in a T75 flask

Subsequent bulk growth. Bulk growth (1x10E8 cells) of a transformed established cell line. Cryopreservation of cell pellet or 20 vials of transformed lymphoblasts. Allow approximately 2-3 weeks for completion.

Subsequent growth. T25 flask (5x10E6 cells) of a transformed cell line. Allow approximately 3-5 days for completion. (Replacement of initial vial)

Subsequent transformation and T25 flask. Transformation of frozen lymphocytes with Epstein-Barr virus. Cryopreservation of 2 vial of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 4 weeks for completion.

Subsequent transformation with bulk growth. Transformation of cryopreserved lymphocytes with Epstein-Barr virus.Bulk growth (1x10E8) of transformed. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquotes. Allow approximately 6-8 weeks for completion.

Subsequent transformation and hold. Transformation of frozen lymphocyteswith Epstein-Barr virus. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 4-5weeks for completion.

Preparation of cell pellet (100,000,000 cells)

Prepare frozen stocks @ 5x10E6 cells each of established cell lines.

Set up cost for Cell Line Distribution when fewer than 5 distribution requests per month are expected

Set up cost for Cell Line Distribution when 5-9 distribution requests per month are expected

Set up cost for Cell Line Distribution when 10-19 distribution requests per month are expected

Cell line deposit: 5 lines, no distribution

User and PI must sign CryoCart/Cryogenic Liquids User Responsibility Agreement prior to use of CryoCart.

Disposal with Certificate of Discard

Customers: Use Dry Ice Request Form at top of page for purchasing

Dry Ice, price per 1 pound

FAIL Attempt for expansion after attempts at service request completion are made 

Supplies needed for modified protocol

FAIL Attempt for Transformation after three attempts are made (or less if requested by researcher) to complete transformation. 

Specimen contaminated upon receipt

Defined dates and times for patient sample processing: including technician monitoring of critical processing time points, refrigerated centrifuge, and aliquoting on ice with snap freezing.

Isolation of RNA from cultured cells by FAF

Mycoplasm Test after completion

DNA Isolation by FAF

PicoGreen as assessed in FAF core.

Purchase of a 9 x 9 Cryo Storage Box, suitable for long term storage.

Purchase of Envelope + Dry Ice for Customer Biospecimen Transport

Retrieval of specimens under vial rate.

Package UN3373 Small 6lb Box 11"x9"x7"

Please email biorepository@jhmi.edu to inquire about purchasing

Package UN3373 Medium 20lb Box 14"x14"14"

Please email biorepository@jhmi.edu to inquire about purchasing

Package UN3373 Large 60lb Box 24"x16"x16"

Please email biorepository@jhmi.edu to inquire about purchasing

FedEx Charges

WorldCourier Charges

Yearly Storage per vial

July 1- June 30 coverage 

Total charge initiated in July for investigators with less than 500 samples.

Quarterly storage per vial

For Investigators with >500 samples

Cost per card