Purchase of a 9 x 9 Cryo Storage Box, suitable for long term storage.

Box Dimenions: 5.0 in x 5.0 in x 2.0 in

Lymphocyte isolation and cryopreservation. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells.Database entry for all aliquots.

Lymphocyte isolation from CPT tubes and cryopreservation. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells.Database entry for all aliquots.

Cryopreservation of additonal vial (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells. Cell count. Viability determination. Database entry for all aliquots.

FAIL Attempt for expansion after attempts at service request completion are made 

Fresh WB Transformation and T25 flask. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells. Transformation of fresh lymphocytes with Epstein-Barr virus.Cryopreservation of 2 vial of (transformed) lymphoblasts @5x10E6cells. Database entry for all aliquotes. Allow 4 weeks for completion.

Fresh WB Transformation and hold. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6 cells. Transformation of fresh lymphocytes with Epstein-Barr virus. Cryopreservation of 4 vials (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow4-5 weeks for completion.

Set up cost for Cell Line Distribution when 5-9 distribution requests per month are expected

Set up cost for Cell Line Distribution when fewer than 5 distribution requests per month are expected

Set up cost for Cell Line Distribution when 10-19 distribution requests per month are expected

Growth of culture for 14 days with visual inspection of contamination

User and PI must sign CryoCart/Cryogenic Liquids User Responsibility Agreement prior to use of CryoCart.

Fresh WB Transformation with bulk growth. Lymphocyte separation. Cell count.Viability determination. Cryopreservation of whole blood (1ml). Cryopresarvation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells.Transformation of fresh lymphocytes with Epstein-Barr virus. Bulk growth (1x10E8) of transformed cells. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 6-8 weeks for completion.

DNA Isolation by FAF

Customers: Use Dry Ice Request Form at top of page for purchasing

Dry Ice, price per 1 pound

Isolation and cryopreservation of plasma. Plasma is separated by centrifugation, recovered in a sterile manner. Cryopreservation of vials (0.5 -1 ml/vial), first 1-5 aliquots included in price (additional vials $1.00/vial), and banked at -80C (additional storage charges apply). Database entry for all aliquots.

Cost per freezer, per month.

Minimum 1 month, 1 freezer. For emergency use only.

Purchase of Envelope + Dry Ice for Customer Biospecimen Transport

Isolation and cryopreservation of sera. Sera is separated by centrifugation, recovered in a sterile manner.  Cryopreservation vial(s) (0.5 -1 ml/vial) vials, first 1-5 aliqots included in price (additional vials $1.00/vial), and banked at -80C (additional storage charges apply). Database entry for all aliquots.

FedEx Charges

Establish primary cells from skin biopsy.  Prepare frozen stocks 4 vials @1x10E6 up to 3x10E6 cells each of established cell lines.  Includes cell count and viability and Mycoplasma test of initial media containing the biopsy.

FAIL Attempt for Transformation after three attempts are made (or less if requested by researcher) to complete transformation. 

Cell culture expansion of established cell line, cryopreservation of replacement vial @5e6 cells/ml and Cell Pellet Preparation. Culture to 10e8 cells suitable for DNA analysis.

Growth of an established adherent cell line in a T175 flask

Growth of an established adherent cell line in a T25 flask

Growth of an established adherent cell line in a T75 flask

Growth of established cell lines in tissue culture flasks. Price per vial 

Growth of established suspension cell lines in T25 flask

Growth of established suspension cell lines in a T75 flask

Preparation of cell pellet (100,000,000 cells)

Subsequent bulk growth. Bulk growth (1x10E8 cells) of a transformed established cell line. Cryopreservation of cell pellet or 20 vials of transformed lymphoblasts. Allow approximately 2-3 weeks for completion.

Subsequent growth. T25 flask (5x10E6 cells) of a transformed cell line. Allow approximately 3-5 days for completion. (Replacement of initial vial)

Subsequent transformation and T25 flask. Transformation of frozen lymphocytes with Epstein-Barr virus. Cryopreservation of 2 vial of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 4 weeks for completion.

Subsequent transformation with bulk growth. Transformation of cryopreserved lymphocytes with Epstein-Barr virus.Bulk growth (1x10E8) of transformed. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquotes. Allow approximately 6-8 weeks for completion.

Subsequent transformation and hold. Transformation of frozen lymphocyteswith Epstein-Barr virus. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 4-5weeks for completion.

Prepare frozen stocks @ 5x10E6 cells each of established cell lines.

Thaw and transformation of lymphocytes w/EBV. Culture to 5x106 cells/ml, suitable for cryopreservation of 2 vial transformed lymphocytes. Allow 4 weeks for completion.

All services listed in (G), plus 2 additional cryopreserved vials of transformed lymphocytes (at 5x106 cells/ml) (Total 4).  Allow 4-5 weeks for completion.

All service in (H), plus culture to 1x108 cells/ml suitable for DNA analysis (cell pellet).  Allow 6-8 weeks for completion.

Specimen contaminated upon receipt

Culture suitable for T25 pick up and cryopreservation of 1 replacement vial (@ 5x10^6 cells/ml).  Allow 3-5 for completion.

Culture to 1x108 cells/ml suitable for DNA analysis (cell pellet) and cryopreservation of 1 replacement vial (@5x10^6 cells/ml).  Allow 2-3 weeks for completion.

Generation and thermal printing of polymer cryovial label measuring either 1.0"x2.75" or 1.0"x2.625". Fits standard Nunc 1.0 - 2.0 mL tubes. Rated down to LN2.

Package UN3373 Large 60lb Box 24"x16"x16"

Please email biorepository@jhmi.edu to inquire about purchasing

Package UN3373 Medium 20lb Box 14"x14"14"

Please email biorepository@jhmi.edu to inquire about purchasing

Cell line deposit: 5 lines, no distribution

Monocyte isolation and cryopreservation. Lymphocyte & Monocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed monocytes @5x10E6cells. Database entry for all aliquots

Mycoplasm Test after completion

Cost per card

Cryogenic storage of boxes in the Johns Hopkins BioBank. Charged quarterly.

Quarterly cryogenic storage for customers with over 500 boxes in storage.

Quarterly Storage per 96-well Microtube Plate. Charged quarterly.

Cryogenic storage of 0.25 mL - 2.0 mL standard cryovials in the Johns Hopkins BioBank. Charged quarterly.

Ambient Storage of Paraffin-embedded tissue. Charged quarterly.

Isolation of RNA from cultured cells by FAF

Package UN3373 Small 6lb Box 11"x9"x7"

Please email biorepository@jhmi.edu to inquire about purchasing

Defined dates and times for patient sample processing: including technician monitoring of critical processing time points, refrigerated centrifuge, and aliquoting on ice with snap freezing.

Disposal with Certificate of Discard

Supplies needed for modified protocol

Sterility testing of retention samples to include aerobic organisms using Tryptic Soy Broth media and anaerobic organisms using Fluid Thioglycollate Medium.  Testing to continue for 14 days at 32.5^C.  Report generated for each sample tested.

Retrieval of specimens under vial rate.

Purchase of a 9 x 9 Cryo Storage Box, suitable for long term storage.

Box Dimenions: 5.0 in x 5.0 in x 2.0 in

Generation and thermal printing of polymer cryovial label measuring either 1.0"x2.75" or 1.0"x2.625". Fits standard Nunc 1.0 - 2.0 mL tubes. Rated down to LN2.

Lymphocyte isolation from CPT tubes and cryopreservation. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells.Database entry for all aliquots.

Cryopreservation of additonal vial (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells. Cell count. Viability determination. Database entry for all aliquots.

Lymphocyte isolation and cryopreservation. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells.Database entry for all aliquots.

Fresh WB Transformation and T25 flask. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells. Transformation of fresh lymphocytes with Epstein-Barr virus.Cryopreservation of 2 vial of (transformed) lymphoblasts @5x10E6cells. Database entry for all aliquotes. Allow 4 weeks for completion.

Fresh WB Transformation and hold. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6 cells. Transformation of fresh lymphocytes with Epstein-Barr virus. Cryopreservation of 4 vials (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow4-5 weeks for completion.

Fresh WB Transformation with bulk growth. Lymphocyte separation. Cell count.Viability determination. Cryopreservation of whole blood (1ml). Cryopresarvation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells.Transformation of fresh lymphocytes with Epstein-Barr virus. Bulk growth (1x10E8) of transformed cells. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 6-8 weeks for completion.

Isolation and cryopreservation of plasma. Plasma is separated by centrifugation, recovered in a sterile manner. Cryopreservation of vials (0.5 -1 ml/vial), first 1-5 aliquots included in price (additional vials $1.00/vial), and banked at -80C (additional storage charges apply). Database entry for all aliquots.

Isolation and cryopreservation of sera. Sera is separated by centrifugation, recovered in a sterile manner.  Cryopreservation vial(s) (0.5 -1 ml/vial) vials, first 1-5 aliqots included in price (additional vials $1.00/vial), and banked at -80C (additional storage charges apply). Database entry for all aliquots.

Thaw and transformation of lymphocytes w/EBV. Culture to 5x106 cells/ml, suitable for cryopreservation of 2 vial transformed lymphocytes. Allow 4 weeks for completion.

All services listed in (G), plus 2 additional cryopreserved vials of transformed lymphocytes (at 5x106 cells/ml) (Total 4).  Allow 4-5 weeks for completion.

All service in (H), plus culture to 1x108 cells/ml suitable for DNA analysis (cell pellet).  Allow 6-8 weeks for completion.

Culture suitable for T25 pick up and cryopreservation of 1 replacement vial (@ 5x10^6 cells/ml).  Allow 3-5 for completion.

Culture to 1x108 cells/ml suitable for DNA analysis (cell pellet) and cryopreservation of 1 replacement vial (@5x10^6 cells/ml).  Allow 2-3 weeks for completion.

Monocyte isolation and cryopreservation. Lymphocyte & Monocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed monocytes @5x10E6cells. Database entry for all aliquots

Growth of culture for 14 days with visual inspection of contamination

Establish primary cells from skin biopsy.  Prepare frozen stocks 4 vials @1x10E6 up to 3x10E6 cells each of established cell lines.  Includes cell count and viability and Mycoplasma test of initial media containing the biopsy.

Cell culture expansion of established cell line, cryopreservation of replacement vial @5e6 cells/ml and Cell Pellet Preparation. Culture to 10e8 cells suitable for DNA analysis.

Growth of an established adherent cell line in a T175 flask

Growth of an established adherent cell line in a T25 flask

Growth of an established adherent cell line in a T75 flask

Growth of established cell lines in tissue culture flasks. Price per vial 

Growth of established suspension cell lines in T25 flask

Growth of established suspension cell lines in a T75 flask

Subsequent bulk growth. Bulk growth (1x10E8 cells) of a transformed established cell line. Cryopreservation of cell pellet or 20 vials of transformed lymphoblasts. Allow approximately 2-3 weeks for completion.

Subsequent growth. T25 flask (5x10E6 cells) of a transformed cell line. Allow approximately 3-5 days for completion. (Replacement of initial vial)

Subsequent transformation and T25 flask. Transformation of frozen lymphocytes with Epstein-Barr virus. Cryopreservation of 2 vial of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 4 weeks for completion.

Subsequent transformation with bulk growth. Transformation of cryopreserved lymphocytes with Epstein-Barr virus.Bulk growth (1x10E8) of transformed. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquotes. Allow approximately 6-8 weeks for completion.

Subsequent transformation and hold. Transformation of frozen lymphocyteswith Epstein-Barr virus. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 4-5weeks for completion.

Preparation of cell pellet (100,000,000 cells)

Prepare frozen stocks @ 5x10E6 cells each of established cell lines.

Set up cost for Cell Line Distribution when fewer than 5 distribution requests per month are expected

Set up cost for Cell Line Distribution when 5-9 distribution requests per month are expected

Set up cost for Cell Line Distribution when 10-19 distribution requests per month are expected

Cell line deposit: 5 lines, no distribution

Sterility testing of retention samples to include aerobic organisms using Tryptic Soy Broth media and anaerobic organisms using Fluid Thioglycollate Medium.  Testing to continue for 14 days at 32.5^C.  Report generated for each sample tested.

User and PI must sign CryoCart/Cryogenic Liquids User Responsibility Agreement prior to use of CryoCart.

Disposal with Certificate of Discard

Customers: Use Dry Ice Request Form at top of page for purchasing

Dry Ice, price per 1 pound

FAIL Attempt for expansion after attempts at service request completion are made 

Supplies needed for modified protocol

FAIL Attempt for Transformation after three attempts are made (or less if requested by researcher) to complete transformation. 

Specimen contaminated upon receipt

Defined dates and times for patient sample processing: including technician monitoring of critical processing time points, refrigerated centrifuge, and aliquoting on ice with snap freezing.

Isolation of RNA from cultured cells by FAF

Mycoplasm Test after completion

DNA Isolation by FAF

Purchase of Envelope + Dry Ice for Customer Biospecimen Transport

Retrieval of specimens under vial rate.

Package UN3373 Small 6lb Box 11"x9"x7"

Please email biorepository@jhmi.edu to inquire about purchasing

Package UN3373 Medium 20lb Box 14"x14"14"

Please email biorepository@jhmi.edu to inquire about purchasing

Package UN3373 Large 60lb Box 24"x16"x16"

Please email biorepository@jhmi.edu to inquire about purchasing

FedEx Charges

Cost per freezer, per month.

Minimum 1 month, 1 freezer. For emergency use only.

Quarterly cryogenic storage for customers with over 500 boxes in storage.

Quarterly Storage per 96-well Microtube Plate. Charged quarterly.

Ambient Storage of Paraffin-embedded tissue. Charged quarterly.

Cryogenic storage of 0.25 mL - 2.0 mL standard cryovials in the Johns Hopkins BioBank. Charged quarterly.

Cryogenic storage of boxes in the Johns Hopkins BioBank. Charged quarterly.

Cost per card