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SKCCC Mass Cytometry Technology Development Center

Overview of Services

 

The SKCCC Flow/Mass Cytometry Core facility strives to provide technical expertise and instrumentation to enhance the quality of interdisciplinary research across JHU.

Flow Cytometry core services include cell sorting, basic training for instrument operation in Flow cytometry core.

 

Mass Cytometry Core provides Consultation, Panel design, Antibody conjugation, Staining, Acquisition and Data analysis.

 

 Service overview: **Please note: All mass cytometry services require consultation and direct coordination with the facility personnel. Please contact Xuan Yuan and Won Jin Ho with inquiries. 

  • Consultation
    • Experimental Design
    • Panel construction and optimization
  • Services
    • Antibody customization
    • Sample staining
    • Multiplex barcoding
    • Suspension mass cytometry (CyTOF) Data acquisition
    • Imaging mass cytometry (IMC) Data acquisition
    • Data analysis (in collaboration with Bioinformatics/Biostats Core)

     Equipment Overview:

  • STANDARD BioTools Helios™ (CyTOF)

 

CyTOF Helios

  • STANDARD BioTools Hyperion+ Imaging System™ (IMC)

 Fluidigm Hyperion Imaging System

 

 

Acknowledgement Policy

This Core is supported through the Sidney Kimmel Cancer Center National Cancer Institute P30 CA006973 Center Grant. 

Cancer Center members should reference CCSG funding (NCI CCSG P30 CA006973) on all cancer center publications where SKCCC Core services are used. 

Acknowledgement of the core facility in all publications which utilized the service of the core is customary and appreciated, as it is used as a metric in determining future funding.

Leadership

Won Jin Ho,  MD | Mass Cytometry Core Director

Xuan Yuan,  MD | Mass Cytometry Core Manager

 Location and hours of operation 

Hours Location

 

 

9:00 AM - 5:00 PM (Mon - Fri)  

 

 

Mass cytometry core in CRB I Room 223

 

Links and Resources

  1. www.bdbiosciences.com
  2. www.biolegend.com
  3. www.thermofisher.com
  4. www.miltenyibiotec.com
  5. www.bio-rad.com
  6. www.fluidigm.com
  7. www.cellsignaling.com

Contacts

Name Role Phone Email Location
Xuan Yuan
Mass Cytometry Core Manager
 
(443) 287-6081
 
yuanxu@jhmi.edu
 
CRB1 1m87
 

Fixed Cost Services


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