SKCCC Mass Cytometry Technology Development Center

**Please note: 

All mass cytometry services require consultation and direct coordination with the facility personnel. Please contact Xuan Yuan and Won Jin Ho with inquiries and  consultation

 

The SKCCC Mass Cytometry Technology Development Center Services include Single-Cell Suspensions are used in Mass Cytometry (CyTOF) and Image Mass Cytometry (IMC).

The SKCCC Mass Cytometry Technology Development Center Provides Consultation, Panel design, Antibody conjugation, Staining, Acquisition and Data analysis.

 

Cytometry by time-of-flight (CyTOF), also known as mass cytometry, is a high-throughput, high-dimensional platform for analyzing single cells.  

 

 Service verview: 

    • Experimental Design- Up to 45 markers in a single assay
    • Panel construction and optimization
    • Antibody customization and validation
    • Sample staining
    • Multiplex barcoding
    • Suspension mass cytometry (CyTOF) Data acquisition
    • Imaging mass cytometry (IMC) Data acquisition
    • Data analysis (in collaboration with Bioinformatics/Biostats Core)

   

 Equipment Overview:

  • STANDARD BioTools Helios™ (CyTOF) and CyTOF XT (Automated cytometry)

 

CyTOF HeliosCyTOF XT | Standard BioTools

  • STANDARD BioTools Hyperion+ Imaging System™ (IMC)  & Hyperion XTi (Automatic cell imaging system & Slide loader can automatic loading and acquisition of up to 40 slides). 

 Fluidigm Hyperion Imaging System

 

 

Acknowledgement Policy

This Core is supported through the Sidney Kimmel Cancer Center National Cancer Institute P30 CA006973 Center Grant. 

Cancer Center members should reference CCSG funding (NCI CCSG P30 CA006973) on all cancer center publications where SKCCC Core services are used. 

Acknowledgement of the core facility in all publications which utilized the service of the core is customary and appreciated, as it is used as a metric in determining future funding.

Leadership

Won Jin Ho,  MD | Mass Cytometry Core Director

Xuan Yuan,  MD | Mass Cytometry Core Manager

 Location and hours of operation 

Hours Location

 

 

9:00 AM - 5:00 PM (Mon - Fri)  

 

 

Mass cytometry core in CRB I Room 223

 

Links and Resources

  1. www.bdbiosciences.com
  2. www.biolegend.com
  3. www.thermofisher.com
  4. www.miltenyibiotec.com
  5. www.bio-rad.com
  6. www.fluidigm.com
  7. www.cellsignaling.com

Contacts

Name Role Phone Email Location
Xuan Yuan
Manager
 
443-287-6081
 
yuanxu@jhmi.edu
 
CRB I Rm 1M87
 
Won Jin Ho
Director
 
410-502-5279
 
www.wjhlab.com
 
CRB I Rm 488
 
Courtney Cannon
Staff
 

 
ccanno19@jh.edu
 
CRB I Rm 1M89
 

Fixed Cost Services


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