ALERT

Important Information Regarding JHU iLab URL Change on 11/9/24


Please be aware that as of 8/3/2024 the URL for the Johns Hopkins University iLab website has changed to https://johnshopkins.ilab.agilent.com.  The temporary URL re-direct will expire on 11/9/24.  Therefore, it is important that you update your saved bookmarks to the new URL prior to this date.  If you encounter any issues with logging into iLab with your JHED ID, please call the Johns Hopkins IT Help Desk at 410-516-HELP (410-516-4357) or submit a Johns Hopkins Help Desk self-service ticket online by accessing https://myit.jh.edu/apps/mfahelpticket.

(hide this warning on this page)

SKCCC Mass Cytometry Technology Development Center

**Please note: 

All mass cytometry services require consultation and direct coordination with the facility personnel. Please contact Xuan Yuan and Won Jin Ho with inquiries and  consultation

 

The SKCCC Mass Cytometry Technology Development Center Services include Single-Cell Suspensions are used in Mass Cytometry (CyTOF) and Image Mass Cytometry (IMC).

The SKCCC Mass Cytometry Technology Development Center Provides Consultation, Panel design, Antibody conjugation, Staining, Acquisition and Data analysis.

 

Cytometry by time-of-flight (CyTOF), also known as mass cytometry, is a high-throughput, high-dimensional platform for analyzing single cells.  

 

 Service verview: 

    • Experimental Design- Up to 45 markers in a single assay
    • Panel construction and optimization
    • Antibody customization and validation
    • Sample staining
    • Multiplex barcoding
    • Suspension mass cytometry (CyTOF) Data acquisition
    • Imaging mass cytometry (IMC) Data acquisition
    • Data analysis (in collaboration with Bioinformatics/Biostats Core)

   

 Equipment Overview:

  • STANDARD BioTools Helios™ (CyTOF)

 

CyTOF Helios

  • STANDARD BioTools Hyperion+ Imaging System™ (IMC)

 Fluidigm Hyperion Imaging System

 

 

Acknowledgement Policy

This Core is supported through the Sidney Kimmel Cancer Center National Cancer Institute P30 CA006973 Center Grant. 

Cancer Center members should reference CCSG funding (NCI CCSG P30 CA006973) on all cancer center publications where SKCCC Core services are used. 

Acknowledgement of the core facility in all publications which utilized the service of the core is customary and appreciated, as it is used as a metric in determining future funding.

Leadership

Won Jin Ho,  MD | Mass Cytometry Core Director

Xuan Yuan,  MD | Mass Cytometry Core Manager

 Location and hours of operation 

Hours Location

 

 

9:00 AM - 5:00 PM (Mon - Fri)  

 

 

Mass cytometry core in CRB I Room 223

 

Links and Resources

  1. www.bdbiosciences.com
  2. www.biolegend.com
  3. www.thermofisher.com
  4. www.miltenyibiotec.com
  5. www.bio-rad.com
  6. www.fluidigm.com
  7. www.cellsignaling.com

Contacts

Name Role Phone Email Location
Xuan Yuan
Manager
 
443-287-6081
 
yuanxu@jhmi.edu
 
CRB I Rm 1M87
 
Won Jin Ho
Director
 
410-502-5279
 
www.wjhlab.com
 
CRB I Rm 488
 
Courtney Cannon
Staff
 

 
ccanno19@jh.edu
 
CRB I Rm 1M89
 

Fixed Cost Services


Search available services: View: by category alphabetically
Consultation (1)